Osmosis Lab Essay Research Paper Osmosis Lab

Osmosis Lab Essay, Research Paper

Osmosis Lab Purpose The purpose of this lab is to determine the concentration of solutes inside of a potato s cell. We also want to know the composition of potato water and stuff . Also the scientific Phenomenon studied of diffusion/ Osmosis. Diffusion is the movement of molecules from an area of higher concentration to an area of lower concentration. Osmosis is process by which water molecules diffuse across a cell membrane from an area of higher concentration to an area of lower concentration.Hypothesis In the potato I think that there will be 8.05% solute. I think that the potato inside the test tube that is labeled #1, that has no sugar inside will gain mass. I believe this because of osmosis, I think that the water will diffuse into the potato because the water it is in has a higher concentration of solute than the potato. In test tube #2 I also think that the potato will gain mass. In the #3 test tube the factor of osmosis will come in and the potato will gain water. In the #4 test tube it will gain even more mass because of the sugar factor. In #5 and #6 they will lose mass, because there is more than 8.05% of sugar in the water. My entire hypothesis is based on the laws of diffusion. Diffusion: The moving of molecules from an area of high concentration to an area of low concentration. In my hypothesis I said that the potato in test tube #1 without any sugars in it would gain mass. This will occur because the potato has a lower concentration of water than water in the test tube, (High Concentration) to inside the potato, (Low concentration) thus the diffusion. So in test tube #4 with 8.05% of sugar will become equilibrium (same amount of diffusion between the two) with the potato. Materials: 1. 5 test tube2. 12 grams of sugar3. Gradated Cylinder4. Aluminum foil 5 1×1in.5. ! beaker6. Scoop7. Balance8. 10 Paper towels9. Masking tape 10in.10. Potato Slice11. Forceps 12. Pen 13. Pen 14. Knife15. Distilled water 1 cup16. Weighing paper 2 1×1in.17. Cork Born18. Stirring Rod Procedure: (Day 1) 1) Put a small piece of tape on each test tube and label them as shown:Group 1 0 1.37 2.73 4.12. 5.63Group 2 0 1.15 2.42 2.78 5.21Group 3 0 1.03 2.14 3.42 4.85Group 4 0 . 75 1.61 3.04 4.52 2) Put 20 ml of water in each test tube.3) Into each test tube put the correct amount of sugar indicated by the test tube s label. (Refer to #1) Calculate the concentration of sugar in each test tube by dividing the amount of sugar by 20 ml. Record this.4) Now cut 5 pieces of potato with the cork borer for consistent potato pieces. Measure and cut the pieces of potato 6-cm in length. Be sure to cut off the pieces with the skin.5) Use the stirring rod on the bottom to get all the sugar dissolved. (Some of the concentrations of sugar very high and may not dissolve)6) One at a time find the mass of potato pieces: put a piece of paper on the balance and find the mass. Put the potato on the paper and find the mass of them together. Subtract the mass of the paper. Record the mass of the potato on your data table in the row for the test tubes. It will go into, and then put the potato into its test tube.7) Put an aluminum foil hat on the test tubes and seal it as well as you can.8) Place the test tubes into a beaker. Using masking tape label the beaker with your names and period.9) Place test beaker on the assigned place on the countertop. Clean up.10) Set up your rough draft of the lab write up. Complete the purpose through procedure.11) Make your predictions about how the mass of the potato spears will change in each sugar solution. Explain why you think certain changes will occur (Look back at the section of diffusion). (Day 2)12) One at a time, using forceps, remove your potato spears from the test tubes.13) Carefully blot it with paper; roll the potato on paper towel to remove excess water from the outside.14) Find the mass of each potato spear using the same method you used on Day 1 and record it.15) Clean your test tubes, and then rinse the forceps. Make your lab space clean. Remove masking tape from test tubes and beakers. Data collection and observations-Formulas: Volume = 3.14r^2L Averages = The sum of all the numbers/how many numbers you added Mass = whatever the balance indicates Percent change in volume = volume of day 2 – day 1 Day 1 * 100 Percent change in mass = mass of day 2 – day 1 Day 1 * 100To calculate the change % of concentration of solute in each test tube:Equation: Amount of sugar x 100 Amount of water Test tube with 0g of sugar: 0g x 100= 0% concentration of solute 20gTest tube with .75g of sugar: .75g x 100= 3.75% 20g Test tube with 1.61g of sugar: 1.61g x 100= 8.05% 20g Test tube with 3.04g of sugar: 3.04g x 100 =15.2% 20g Test tube with 4.52g of sugar: 4.52g x 100= 22.6% 20gWeigh in grams: Potato Paper Total Potato went into of solute 1 1.12 2.45 1.33 0% 2 1.12 2.41 1.29 3.75%

3 1.12 2.40 1.28 8.05% 4 1.12 2.35 1.23 15.2% 5 1.12 2.35 1.23 22.6%Fig. 1- this chart shows the weight of the potato and the patter used to weigh it, as well as where each potato went into of solute.(Day 2)Potato mass in grams: Potato in Mass No sugar 1.94 .75 1.651.61 1.543.04 1.114.52 1.00Fig. 1- this chart shows the potatoes mass gathered in day two. To calculate percent change in massEquation: Mass Day 2 – Mass Day 1 x 100 Mass Day 1 Potato in 0% solute: 1.94 – 1.33 x 100 = 45.9% 1.33Potato in 3.75% solute: 1.65 – 1.29 x 100 = 27.9% 1.29Potato in 8.05% solu